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KMID : 0355220130380040291
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Journal of Korean Academy Oral Medicine
2013 Volume.38 No. 4 p.291 ~ p.298
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Apo Taq expression on salivary glands by the restraint stress in Rat
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Cho Sung-Guk
Kang Soo-Kyung
Auh Q-Schick
Chun Yang-Hyun
Hong Jung-Pyo
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Abstract
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On this study, we treated rats with restraint stress, and observed the changes with an optical microscope. Within the salivary gland tissue, we measured cell apoptosis cycle evaluation which show positive reaction on TUNEL assay, and compared within the groups.
For this study, 18 rats were divided into 3 groups; 1) 2 rats of group Ⅰ were selected as a normal control. 2) 2 rats of group Ⅱ, as a experimental control were placed in the restraint cone for 2 hours 3) 14 rats of group Ⅲ were placed in the restraint cone for 2 hours once a day. The rats were sacrificed immediately (group Ⅱ, as a experimental control), 1, 2, 3, 4, 5, 6 and 7days after application of the stress and the both parotid glands were excised.
The conclusions follow.
1. 5 days after giving an confining stress to the parotid gland of Rats, we can observe the hypotropy and pus and inflammation of Rat parotid gland acinar cells, and after 7 days, we can see a cell apoptosis.
2. Through the In situ DNA end labeling assay and TUNEL dye, on serous glands, benign tumor cell increased with statistically significant result after 5 days from confining stress. And the index shows maximum value on 7th days, which is same result with histological opinion.
Therefore, our study shows that a cell apoptosis can be induced by restraint stress on salivary gland tissue, and we think more study should be accomplished about the cell signaling pathway in the future.
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KEYWORD
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Apoptosis, Restraint stress, Salivary glands
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